Recombinant:Article Title: Integrin-dependent neutrophil slowing reduces lung perfusion and supports metastasis in a model of breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CD3-AF488 (clone 17A2) BioLegend Cat# 100210; RRID:AB_389301 CD68-AF594 (clone FA-11) BioLegend Cat# 137020; RRID:AB_2563305 CD31-BV421 (clone 390) BioLegend Cat# 102424; RRID:AB_2650892 CD31-AF488 (clone 390) BioLegend Cat# 102414; RRID:AB_493408 CD8-AF488 (clone 53-6.7) BioLegend Cat# 100723; RRID:AB_389304 Ly6G-AF647 (clone 1A8) BioLegend Cat# 127610; RRID:AB_1134159 CD11c-AF594 (clone N418) BioLegend Cat# 117346; RRID:AB_2563323 CD45-Spark NIR 685 (clone 30-F11) BioLegend Cat# 103168; RRID:AB_2832301 Ly6G-DyLight 550 (clone 1A8) Novus Biologicals Cat# NBP2-81053R; RRID:AB_3411066 Ly6G (clone 1A8) BioXcell Cat# BE0075-1; RRID:AB_1107721 Chemicals, peptides, and recombinant proteins Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Scientific Cat# 28908 Ethanol VWR Cat# 20821.365 Xylene Genta Cat# 7879 ER2 retrieval buffer Leica Biosystems Cat# AR9640 DAB substrate kit Leica Biosystems Cat# D9263 Low-melting point agarose Thermo Fisher Scientific Cat# J66319.22 Mouse recombinant G-CSF STEMCELL Technologies Cat# 78014 and 78014.2 DNase I Merck Cat# 10104159001 Liberase TM Roche Cat# 5401127001 Liberase TH Roche Cat# 5401151001 HBSS Thermo Fisher Scientific Cat# 14175095 BSA Merck Cat# A7906-500G EDTA Merck Cat# 324503-100GM DAPI Merck Cat# D1306 10% NBF VWR Cat# 11699455 DISCOVERY Omnimap anti-Gt Multimer Roche Cat# 06607233001 FcR Blocking Reagent, mouse Miltenyi Biotec Cat# 130-092-575 Red Blood Cell Lysis Solution Miltenyi Biotec Cat# 130-094-183 BD Horizon Brilliant Stain Buffer BD Biosciences Cat# 566349 Zombie NIR Fixable Viability Kit BioLegend Cat# 423106 Zombie Yellow Fixable Viability Kit BioLegend Cat# 423104 Zombie Green Fixable Viability Kit BioLegend Cat# 423112 CellTrace Far-Red Invitrogen Cat# C34564 CellTrace Yellow Invitrogen Cat# C34573 FBS (Fetal Bovine Serum) Thermo Fisher Scientific Cat# A5256701 BSA (Bovine Serum Albumin) Merck Cat# A3059 RPMI 1640 medium Thermo Fisher Scientific Cat# 31870025 Fetal Bovine Serum Thermo Fisher Scientific Cat# A5256701 Normal goat serum (NGS) Merck Cat# G9023 Triton X-100 Merck Cat# T8787 1% w/v Sodium Azide Solution Severn Biotech Cat# 40-2000-01 DAPI Thermo Fisher Scientific Cat# 62247 Hoechst 33342 Thermo Fisher Scientific Cat# 62249 FluoSpheres blue-fluorescent microspheres, 10 μm diameter Thermo Fisher Scientific Cat# F8829 FluoSpheres yellow-green sulfate microspheres, 4.0 μm diameter Thermo Fisher Scientific Cat# F8859 (Continued on next page) 14 Cell Reports 45, 117101, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER FluoSpheres red microspheres, 0.02 μm diameter Thermo Fisher Scientific Cat# F8786 CountBright counting microbeads Invitrogen Cat# C36950 AccuCount Fluorescent Particles, 7.5 μm Spherotech Cat# ACFP-70-10 UltraComp eBeads Compensation Beads Thermo Fisher Scientific Cat# 01-2222-42 NH4Cl Merck Cat# A9434 KHCO3 Merck Cat# 60339 Na2EDTA Merck Cat# E5134 Ce3D BioLegend Cat# 427704 DMEM Thermo Fisher Scientific Cat# 31053028 Critical commercial assays ROS-ID ROS/RNS detection kit Enzo Life Sciences Cat# ENZ-51001 Experimental models: Organisms/strains Mouse: FVB/N (♀, 8–15 weeks) Charles River Laboratories N/A Mouse: CatchupIVM-red (Ly6G∧Cretom/+ ROSA26∧tdtom/+) Gift Matthias Gunzer (University Duisburg–Essen, Essen, Germany) N/A Mouse: K14-Cre;Trp53∧F/F (FVB/N background) Gift Jos Jonkers (Netherlands Cancer Institute) N/A Mouse: Rag1 KO FVB/NJ (♀, 6–10 weeks) Gift J. Huelsken (EPFL, Lausanne, Switzerland) N/A Software and algorithms Imaris Bitplane/Oxford Instruments https://imaris.oxinst.com FlowJo BD Biosciences https://www.flowjo.com GraphPad Prism v9.0 and v10.0 GraphPad Software https://www.graphpad.com Zen Carl Zeiss https://www.zeiss.com QuPath Bankhead et al. https://qupath.github.io FacsDiva BD Biosciences N/A Fiji Schindelin et al.47 https://imagej.net/software/fiji/ Correct 3D Drift Parslow et al.48 N/A Other BD LSRFortessa flow cytometer BD Biosciences N/A Zeiss LSM 880 confocal with 32-channel GaAsP detector Carl Zeiss N/A Opera Phenix spinning disk microscope PerkinElmer N/A Vibratome Campden Instruments Cat# 5100mz Stainless Steel Blades Campden Instruments Cat# 7550-1-SS Bond Rx autostainer Leica Microsystems SN#3498136, SN# 3219653 SN# 3219768 Leica Aperio AT2 slide scanner Leica Microsystems SN# 7879 Ventana Discovery Roche N/A Axioscan Zeiss N/A Frame-Seal in situ PCR and Hybridization Slide Chambers Bio-Rad Cat# SLF0601 Microscope Slides VWR Cat# 631-0117 Coverslips Marienfield Cat# μ-Slide 8 Well High Ibidi Cat# 80806 Nunc Lab-Tek Chambered Coverglass Thermo Fisher Scientific Cat# 155411 Cell Reports 45, 117101, March 24, 2026 15
Blocking Assay:Article Title: Integrin-dependent neutrophil slowing reduces lung perfusion and supports metastasis in a model of breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CD3-AF488 (clone 17A2) BioLegend Cat# 100210; RRID:AB_389301 CD68-AF594 (clone FA-11) BioLegend Cat# 137020; RRID:AB_2563305 CD31-BV421 (clone 390) BioLegend Cat# 102424; RRID:AB_2650892 CD31-AF488 (clone 390) BioLegend Cat# 102414; RRID:AB_493408 CD8-AF488 (clone 53-6.7) BioLegend Cat# 100723; RRID:AB_389304 Ly6G-AF647 (clone 1A8) BioLegend Cat# 127610; RRID:AB_1134159 CD11c-AF594 (clone N418) BioLegend Cat# 117346; RRID:AB_2563323 CD45-Spark NIR 685 (clone 30-F11) BioLegend Cat# 103168; RRID:AB_2832301 Ly6G-DyLight 550 (clone 1A8) Novus Biologicals Cat# NBP2-81053R; RRID:AB_3411066 Ly6G (clone 1A8) BioXcell Cat# BE0075-1; RRID:AB_1107721 Chemicals, peptides, and recombinant proteins Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Scientific Cat# 28908 Ethanol VWR Cat# 20821.365 Xylene Genta Cat# 7879 ER2 retrieval buffer Leica Biosystems Cat# AR9640 DAB substrate kit Leica Biosystems Cat# D9263 Low-melting point agarose Thermo Fisher Scientific Cat# J66319.22 Mouse recombinant G-CSF STEMCELL Technologies Cat# 78014 and 78014.2 DNase I Merck Cat# 10104159001 Liberase TM Roche Cat# 5401127001 Liberase TH Roche Cat# 5401151001 HBSS Thermo Fisher Scientific Cat# 14175095 BSA Merck Cat# A7906-500G EDTA Merck Cat# 324503-100GM DAPI Merck Cat# D1306 10% NBF VWR Cat# 11699455 DISCOVERY Omnimap anti-Gt Multimer Roche Cat# 06607233001 FcR Blocking Reagent, mouse Miltenyi Biotec Cat# 130-092-575 Red Blood Cell Lysis Solution Miltenyi Biotec Cat# 130-094-183 BD Horizon Brilliant Stain Buffer BD Biosciences Cat# 566349 Zombie NIR Fixable Viability Kit BioLegend Cat# 423106 Zombie Yellow Fixable Viability Kit BioLegend Cat# 423104 Zombie Green Fixable Viability Kit BioLegend Cat# 423112 CellTrace Far-Red Invitrogen Cat# C34564 CellTrace Yellow Invitrogen Cat# C34573 FBS (Fetal Bovine Serum) Thermo Fisher Scientific Cat# A5256701 BSA (Bovine Serum Albumin) Merck Cat# A3059 RPMI 1640 medium Thermo Fisher Scientific Cat# 31870025 Fetal Bovine Serum Thermo Fisher Scientific Cat# A5256701 Normal goat serum (NGS) Merck Cat# G9023 Triton X-100 Merck Cat# T8787 1% w/v Sodium Azide Solution Severn Biotech Cat# 40-2000-01 DAPI Thermo Fisher Scientific Cat# 62247 Hoechst 33342 Thermo Fisher Scientific Cat# 62249 FluoSpheres blue-fluorescent microspheres, 10 μm diameter Thermo Fisher Scientific Cat# F8829 FluoSpheres yellow-green sulfate microspheres, 4.0 μm diameter Thermo Fisher Scientific Cat# F8859 (Continued on next page) 14 Cell Reports 45, 117101, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER FluoSpheres red microspheres, 0.02 μm diameter Thermo Fisher Scientific Cat# F8786 CountBright counting microbeads Invitrogen Cat# C36950 AccuCount Fluorescent Particles, 7.5 μm Spherotech Cat# ACFP-70-10 UltraComp eBeads Compensation Beads Thermo Fisher Scientific Cat# 01-2222-42 NH4Cl Merck Cat# A9434 KHCO3 Merck Cat# 60339 Na2EDTA Merck Cat# E5134 Ce3D BioLegend Cat# 427704 DMEM Thermo Fisher Scientific Cat# 31053028 Critical commercial assays ROS-ID ROS/RNS detection kit Enzo Life Sciences Cat# ENZ-51001 Experimental models: Organisms/strains Mouse: FVB/N (♀, 8–15 weeks) Charles River Laboratories N/A Mouse: CatchupIVM-red (Ly6G∧Cretom/+ ROSA26∧tdtom/+) Gift Matthias Gunzer (University Duisburg–Essen, Essen, Germany) N/A Mouse: K14-Cre;Trp53∧F/F (FVB/N background) Gift Jos Jonkers (Netherlands Cancer Institute) N/A Mouse: Rag1 KO FVB/NJ (♀, 6–10 weeks) Gift J. Huelsken (EPFL, Lausanne, Switzerland) N/A Software and algorithms Imaris Bitplane/Oxford Instruments https://imaris.oxinst.com FlowJo BD Biosciences https://www.flowjo.com GraphPad Prism v9.0 and v10.0 GraphPad Software https://www.graphpad.com Zen Carl Zeiss https://www.zeiss.com QuPath Bankhead et al. https://qupath.github.io FacsDiva BD Biosciences N/A Fiji Schindelin et al.47 https://imagej.net/software/fiji/ Correct 3D Drift Parslow et al.48 N/A Other BD LSRFortessa flow cytometer BD Biosciences N/A Zeiss LSM 880 confocal with 32-channel GaAsP detector Carl Zeiss N/A Opera Phenix spinning disk microscope PerkinElmer N/A Vibratome Campden Instruments Cat# 5100mz Stainless Steel Blades Campden Instruments Cat# 7550-1-SS Bond Rx autostainer Leica Microsystems SN#3498136, SN# 3219653 SN# 3219768 Leica Aperio AT2 slide scanner Leica Microsystems SN# 7879 Ventana Discovery Roche N/A Axioscan Zeiss N/A Frame-Seal in situ PCR and Hybridization Slide Chambers Bio-Rad Cat# SLF0601 Microscope Slides VWR Cat# 631-0117 Coverslips Marienfield Cat# μ-Slide 8 Well High Ibidi Cat# 80806 Nunc Lab-Tek Chambered Coverglass Thermo Fisher Scientific Cat# 155411 Cell Reports 45, 117101, March 24, 2026 15
Red Blood Cell Lysis:Article Title: Integrin-dependent neutrophil slowing reduces lung perfusion and supports metastasis in a model of breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CD3-AF488 (clone 17A2) BioLegend Cat# 100210; RRID:AB_389301 CD68-AF594 (clone FA-11) BioLegend Cat# 137020; RRID:AB_2563305 CD31-BV421 (clone 390) BioLegend Cat# 102424; RRID:AB_2650892 CD31-AF488 (clone 390) BioLegend Cat# 102414; RRID:AB_493408 CD8-AF488 (clone 53-6.7) BioLegend Cat# 100723; RRID:AB_389304 Ly6G-AF647 (clone 1A8) BioLegend Cat# 127610; RRID:AB_1134159 CD11c-AF594 (clone N418) BioLegend Cat# 117346; RRID:AB_2563323 CD45-Spark NIR 685 (clone 30-F11) BioLegend Cat# 103168; RRID:AB_2832301 Ly6G-DyLight 550 (clone 1A8) Novus Biologicals Cat# NBP2-81053R; RRID:AB_3411066 Ly6G (clone 1A8) BioXcell Cat# BE0075-1; RRID:AB_1107721 Chemicals, peptides, and recombinant proteins Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Scientific Cat# 28908 Ethanol VWR Cat# 20821.365 Xylene Genta Cat# 7879 ER2 retrieval buffer Leica Biosystems Cat# AR9640 DAB substrate kit Leica Biosystems Cat# D9263 Low-melting point agarose Thermo Fisher Scientific Cat# J66319.22 Mouse recombinant G-CSF STEMCELL Technologies Cat# 78014 and 78014.2 DNase I Merck Cat# 10104159001 Liberase TM Roche Cat# 5401127001 Liberase TH Roche Cat# 5401151001 HBSS Thermo Fisher Scientific Cat# 14175095 BSA Merck Cat# A7906-500G EDTA Merck Cat# 324503-100GM DAPI Merck Cat# D1306 10% NBF VWR Cat# 11699455 DISCOVERY Omnimap anti-Gt Multimer Roche Cat# 06607233001 FcR Blocking Reagent, mouse Miltenyi Biotec Cat# 130-092-575 Red Blood Cell Lysis Solution Miltenyi Biotec Cat# 130-094-183 BD Horizon Brilliant Stain Buffer BD Biosciences Cat# 566349 Zombie NIR Fixable Viability Kit BioLegend Cat# 423106 Zombie Yellow Fixable Viability Kit BioLegend Cat# 423104 Zombie Green Fixable Viability Kit BioLegend Cat# 423112 CellTrace Far-Red Invitrogen Cat# C34564 CellTrace Yellow Invitrogen Cat# C34573 FBS (Fetal Bovine Serum) Thermo Fisher Scientific Cat# A5256701 BSA (Bovine Serum Albumin) Merck Cat# A3059 RPMI 1640 medium Thermo Fisher Scientific Cat# 31870025 Fetal Bovine Serum Thermo Fisher Scientific Cat# A5256701 Normal goat serum (NGS) Merck Cat# G9023 Triton X-100 Merck Cat# T8787 1% w/v Sodium Azide Solution Severn Biotech Cat# 40-2000-01 DAPI Thermo Fisher Scientific Cat# 62247 Hoechst 33342 Thermo Fisher Scientific Cat# 62249 FluoSpheres blue-fluorescent microspheres, 10 μm diameter Thermo Fisher Scientific Cat# F8829 FluoSpheres yellow-green sulfate microspheres, 4.0 μm diameter Thermo Fisher Scientific Cat# F8859 (Continued on next page) 14 Cell Reports 45, 117101, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER FluoSpheres red microspheres, 0.02 μm diameter Thermo Fisher Scientific Cat# F8786 CountBright counting microbeads Invitrogen Cat# C36950 AccuCount Fluorescent Particles, 7.5 μm Spherotech Cat# ACFP-70-10 UltraComp eBeads Compensation Beads Thermo Fisher Scientific Cat# 01-2222-42 NH4Cl Merck Cat# A9434 KHCO3 Merck Cat# 60339 Na2EDTA Merck Cat# E5134 Ce3D BioLegend Cat# 427704 DMEM Thermo Fisher Scientific Cat# 31053028 Critical commercial assays ROS-ID ROS/RNS detection kit Enzo Life Sciences Cat# ENZ-51001 Experimental models: Organisms/strains Mouse: FVB/N (♀, 8–15 weeks) Charles River Laboratories N/A Mouse: CatchupIVM-red (Ly6G∧Cretom/+ ROSA26∧tdtom/+) Gift Matthias Gunzer (University Duisburg–Essen, Essen, Germany) N/A Mouse: K14-Cre;Trp53∧F/F (FVB/N background) Gift Jos Jonkers (Netherlands Cancer Institute) N/A Mouse: Rag1 KO FVB/NJ (♀, 6–10 weeks) Gift J. Huelsken (EPFL, Lausanne, Switzerland) N/A Software and algorithms Imaris Bitplane/Oxford Instruments https://imaris.oxinst.com FlowJo BD Biosciences https://www.flowjo.com GraphPad Prism v9.0 and v10.0 GraphPad Software https://www.graphpad.com Zen Carl Zeiss https://www.zeiss.com QuPath Bankhead et al. https://qupath.github.io FacsDiva BD Biosciences N/A Fiji Schindelin et al.47 https://imagej.net/software/fiji/ Correct 3D Drift Parslow et al.48 N/A Other BD LSRFortessa flow cytometer BD Biosciences N/A Zeiss LSM 880 confocal with 32-channel GaAsP detector Carl Zeiss N/A Opera Phenix spinning disk microscope PerkinElmer N/A Vibratome Campden Instruments Cat# 5100mz Stainless Steel Blades Campden Instruments Cat# 7550-1-SS Bond Rx autostainer Leica Microsystems SN#3498136, SN# 3219653 SN# 3219768 Leica Aperio AT2 slide scanner Leica Microsystems SN# 7879 Ventana Discovery Roche N/A Axioscan Zeiss N/A Frame-Seal in situ PCR and Hybridization Slide Chambers Bio-Rad Cat# SLF0601 Microscope Slides VWR Cat# 631-0117 Coverslips Marienfield Cat# μ-Slide 8 Well High Ibidi Cat# 80806 Nunc Lab-Tek Chambered Coverglass Thermo Fisher Scientific Cat# 155411 Cell Reports 45, 117101, March 24, 2026 15
Staining:Article Title: Integrin-dependent neutrophil slowing reduces lung perfusion and supports metastasis in a model of breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CD3-AF488 (clone 17A2) BioLegend Cat# 100210; RRID:AB_389301 CD68-AF594 (clone FA-11) BioLegend Cat# 137020; RRID:AB_2563305 CD31-BV421 (clone 390) BioLegend Cat# 102424; RRID:AB_2650892 CD31-AF488 (clone 390) BioLegend Cat# 102414; RRID:AB_493408 CD8-AF488 (clone 53-6.7) BioLegend Cat# 100723; RRID:AB_389304 Ly6G-AF647 (clone 1A8) BioLegend Cat# 127610; RRID:AB_1134159 CD11c-AF594 (clone N418) BioLegend Cat# 117346; RRID:AB_2563323 CD45-Spark NIR 685 (clone 30-F11) BioLegend Cat# 103168; RRID:AB_2832301 Ly6G-DyLight 550 (clone 1A8) Novus Biologicals Cat# NBP2-81053R; RRID:AB_3411066 Ly6G (clone 1A8) BioXcell Cat# BE0075-1; RRID:AB_1107721 Chemicals, peptides, and recombinant proteins Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Scientific Cat# 28908 Ethanol VWR Cat# 20821.365 Xylene Genta Cat# 7879 ER2 retrieval buffer Leica Biosystems Cat# AR9640 DAB substrate kit Leica Biosystems Cat# D9263 Low-melting point agarose Thermo Fisher Scientific Cat# J66319.22 Mouse recombinant G-CSF STEMCELL Technologies Cat# 78014 and 78014.2 DNase I Merck Cat# 10104159001 Liberase TM Roche Cat# 5401127001 Liberase TH Roche Cat# 5401151001 HBSS Thermo Fisher Scientific Cat# 14175095 BSA Merck Cat# A7906-500G EDTA Merck Cat# 324503-100GM DAPI Merck Cat# D1306 10% NBF VWR Cat# 11699455 DISCOVERY Omnimap anti-Gt Multimer Roche Cat# 06607233001 FcR Blocking Reagent, mouse Miltenyi Biotec Cat# 130-092-575 Red Blood Cell Lysis Solution Miltenyi Biotec Cat# 130-094-183 BD Horizon Brilliant Stain Buffer BD Biosciences Cat# 566349 Zombie NIR Fixable Viability Kit BioLegend Cat# 423106 Zombie Yellow Fixable Viability Kit BioLegend Cat# 423104 Zombie Green Fixable Viability Kit BioLegend Cat# 423112 CellTrace Far-Red Invitrogen Cat# C34564 CellTrace Yellow Invitrogen Cat# C34573 FBS (Fetal Bovine Serum) Thermo Fisher Scientific Cat# A5256701 BSA (Bovine Serum Albumin) Merck Cat# A3059 RPMI 1640 medium Thermo Fisher Scientific Cat# 31870025 Fetal Bovine Serum Thermo Fisher Scientific Cat# A5256701 Normal goat serum (NGS) Merck Cat# G9023 Triton X-100 Merck Cat# T8787 1% w/v Sodium Azide Solution Severn Biotech Cat# 40-2000-01 DAPI Thermo Fisher Scientific Cat# 62247 Hoechst 33342 Thermo Fisher Scientific Cat# 62249 FluoSpheres blue-fluorescent microspheres, 10 μm diameter Thermo Fisher Scientific Cat# F8829 FluoSpheres yellow-green sulfate microspheres, 4.0 μm diameter Thermo Fisher Scientific Cat# F8859 (Continued on next page) 14 Cell Reports 45, 117101, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER FluoSpheres red microspheres, 0.02 μm diameter Thermo Fisher Scientific Cat# F8786 CountBright counting microbeads Invitrogen Cat# C36950 AccuCount Fluorescent Particles, 7.5 μm Spherotech Cat# ACFP-70-10 UltraComp eBeads Compensation Beads Thermo Fisher Scientific Cat# 01-2222-42 NH4Cl Merck Cat# A9434 KHCO3 Merck Cat# 60339 Na2EDTA Merck Cat# E5134 Ce3D BioLegend Cat# 427704 DMEM Thermo Fisher Scientific Cat# 31053028 Critical commercial assays ROS-ID ROS/RNS detection kit Enzo Life Sciences Cat# ENZ-51001 Experimental models: Organisms/strains Mouse: FVB/N (♀, 8–15 weeks) Charles River Laboratories N/A Mouse: CatchupIVM-red (Ly6G∧Cretom/+ ROSA26∧tdtom/+) Gift Matthias Gunzer (University Duisburg–Essen, Essen, Germany) N/A Mouse: K14-Cre;Trp53∧F/F (FVB/N background) Gift Jos Jonkers (Netherlands Cancer Institute) N/A Mouse: Rag1 KO FVB/NJ (♀, 6–10 weeks) Gift J. Huelsken (EPFL, Lausanne, Switzerland) N/A Software and algorithms Imaris Bitplane/Oxford Instruments https://imaris.oxinst.com FlowJo BD Biosciences https://www.flowjo.com GraphPad Prism v9.0 and v10.0 GraphPad Software https://www.graphpad.com Zen Carl Zeiss https://www.zeiss.com QuPath Bankhead et al. https://qupath.github.io FacsDiva BD Biosciences N/A Fiji Schindelin et al.47 https://imagej.net/software/fiji/ Correct 3D Drift Parslow et al.48 N/A Other BD LSRFortessa flow cytometer BD Biosciences N/A Zeiss LSM 880 confocal with 32-channel GaAsP detector Carl Zeiss N/A Opera Phenix spinning disk microscope PerkinElmer N/A Vibratome Campden Instruments Cat# 5100mz Stainless Steel Blades Campden Instruments Cat# 7550-1-SS Bond Rx autostainer Leica Microsystems SN#3498136, SN# 3219653 SN# 3219768 Leica Aperio AT2 slide scanner Leica Microsystems SN# 7879 Ventana Discovery Roche N/A Axioscan Zeiss N/A Frame-Seal in situ PCR and Hybridization Slide Chambers Bio-Rad Cat# SLF0601 Microscope Slides VWR Cat# 631-0117 Coverslips Marienfield Cat# μ-Slide 8 Well High Ibidi Cat# 80806 Nunc Lab-Tek Chambered Coverglass Thermo Fisher Scientific Cat# 155411 Cell Reports 45, 117101, March 24, 2026 15
Next-Generation Sequencing:Article Title: Integrin-dependent neutrophil slowing reduces lung perfusion and supports metastasis in a model of breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CD3-AF488 (clone 17A2) BioLegend Cat# 100210; RRID:AB_389301 CD68-AF594 (clone FA-11) BioLegend Cat# 137020; RRID:AB_2563305 CD31-BV421 (clone 390) BioLegend Cat# 102424; RRID:AB_2650892 CD31-AF488 (clone 390) BioLegend Cat# 102414; RRID:AB_493408 CD8-AF488 (clone 53-6.7) BioLegend Cat# 100723; RRID:AB_389304 Ly6G-AF647 (clone 1A8) BioLegend Cat# 127610; RRID:AB_1134159 CD11c-AF594 (clone N418) BioLegend Cat# 117346; RRID:AB_2563323 CD45-Spark NIR 685 (clone 30-F11) BioLegend Cat# 103168; RRID:AB_2832301 Ly6G-DyLight 550 (clone 1A8) Novus Biologicals Cat# NBP2-81053R; RRID:AB_3411066 Ly6G (clone 1A8) BioXcell Cat# BE0075-1; RRID:AB_1107721 Chemicals, peptides, and recombinant proteins Pierce 16% Formaldehyde (w/v), Methanol-free Thermo Fisher Scientific Cat# 28908 Ethanol VWR Cat# 20821.365 Xylene Genta Cat# 7879 ER2 retrieval buffer Leica Biosystems Cat# AR9640 DAB substrate kit Leica Biosystems Cat# D9263 Low-melting point agarose Thermo Fisher Scientific Cat# J66319.22 Mouse recombinant G-CSF STEMCELL Technologies Cat# 78014 and 78014.2 DNase I Merck Cat# 10104159001 Liberase TM Roche Cat# 5401127001 Liberase TH Roche Cat# 5401151001 HBSS Thermo Fisher Scientific Cat# 14175095 BSA Merck Cat# A7906-500G EDTA Merck Cat# 324503-100GM DAPI Merck Cat# D1306 10% NBF VWR Cat# 11699455 DISCOVERY Omnimap anti-Gt Multimer Roche Cat# 06607233001 FcR Blocking Reagent, mouse Miltenyi Biotec Cat# 130-092-575 Red Blood Cell Lysis Solution Miltenyi Biotec Cat# 130-094-183 BD Horizon Brilliant Stain Buffer BD Biosciences Cat# 566349 Zombie NIR Fixable Viability Kit BioLegend Cat# 423106 Zombie Yellow Fixable Viability Kit BioLegend Cat# 423104 Zombie Green Fixable Viability Kit BioLegend Cat# 423112 CellTrace Far-Red Invitrogen Cat# C34564 CellTrace Yellow Invitrogen Cat# C34573 FBS (Fetal Bovine Serum) Thermo Fisher Scientific Cat# A5256701 BSA (Bovine Serum Albumin) Merck Cat# A3059 RPMI 1640 medium Thermo Fisher Scientific Cat# 31870025 Fetal Bovine Serum Thermo Fisher Scientific Cat# A5256701 Normal goat serum (NGS) Merck Cat# G9023 Triton X-100 Merck Cat# T8787 1% w/v Sodium Azide Solution Severn Biotech Cat# 40-2000-01 DAPI Thermo Fisher Scientific Cat# 62247 Hoechst 33342 Thermo Fisher Scientific Cat# 62249 FluoSpheres blue-fluorescent microspheres, 10 μm diameter Thermo Fisher Scientific Cat# F8829 FluoSpheres yellow-green sulfate microspheres, 4.0 μm diameter Thermo Fisher Scientific Cat# F8859 (Continued on next page) 14 Cell Reports 45, 117101, March 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER FluoSpheres red microspheres, 0.02 μm diameter Thermo Fisher Scientific Cat# F8786 CountBright counting microbeads Invitrogen Cat# C36950 AccuCount Fluorescent Particles, 7.5 μm Spherotech Cat# ACFP-70-10 UltraComp eBeads Compensation Beads Thermo Fisher Scientific Cat# 01-2222-42 NH4Cl Merck Cat# A9434 KHCO3 Merck Cat# 60339 Na2EDTA Merck Cat# E5134 Ce3D BioLegend Cat# 427704 DMEM Thermo Fisher Scientific Cat# 31053028 Critical commercial assays ROS-ID ROS/RNS detection kit Enzo Life Sciences Cat# ENZ-51001 Experimental models: Organisms/strains Mouse: FVB/N (♀, 8–15 weeks) Charles River Laboratories N/A Mouse: CatchupIVM-red (Ly6G∧Cretom/+ ROSA26∧tdtom/+) Gift Matthias Gunzer (University Duisburg–Essen, Essen, Germany) N/A Mouse: K14-Cre;Trp53∧F/F (FVB/N background) Gift Jos Jonkers (Netherlands Cancer Institute) N/A Mouse: Rag1 KO FVB/NJ (♀, 6–10 weeks) Gift J. Huelsken (EPFL, Lausanne, Switzerland) N/A Software and algorithms Imaris Bitplane/Oxford Instruments https://imaris.oxinst.com FlowJo BD Biosciences https://www.flowjo.com GraphPad Prism v9.0 and v10.0 GraphPad Software https://www.graphpad.com Zen Carl Zeiss https://www.zeiss.com QuPath Bankhead et al. https://qupath.github.io FacsDiva BD Biosciences N/A Fiji Schindelin et al.47 https://imagej.net/software/fiji/ Correct 3D Drift Parslow et al.48 N/A Other BD LSRFortessa flow cytometer BD Biosciences N/A Zeiss LSM 880 confocal with 32-channel GaAsP detector Carl Zeiss N/A Opera Phenix spinning disk microscope PerkinElmer N/A Vibratome Campden Instruments Cat# 5100mz Stainless Steel Blades Campden Instruments Cat# 7550-1-SS Bond Rx autostainer Leica Microsystems SN#3498136, SN# 3219653 SN# 3219768 Leica Aperio AT2 slide scanner Leica Microsystems SN# 7879 Ventana Discovery Roche N/A Axioscan Zeiss N/A Frame-Seal in situ PCR and Hybridization Slide Chambers Bio-Rad Cat# SLF0601 Microscope Slides VWR Cat# 631-0117 Coverslips Marienfield Cat# μ-Slide 8 Well High Ibidi Cat# 80806 Nunc Lab-Tek Chambered Coverglass Thermo Fisher Scientific Cat# 155411 Cell Reports 45, 117101, March 24, 2026 15
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